Научная визуализация, 2026, том 18, номер 2, страницы 24 - 33, DOI: 10.26583/sv.18.2.03
Visualization of Lung Vessels in Optically Cleared Specimens Using Second Harmonic Generation
Авторы: S.A. Portnov1,A, S.S. Shalyapin2,A, A.O. Bogorodskiy3,A, V.I. Borshchevskiy4,B, M.A. Shevchenko5,C
A National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
B Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology
C Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS
1 ORCID: 0009-0000-7978-9486, portnov.hb @gmail.com
2 ORCID: 0009-0005-6101-3133, shalyapin956@gmail.com
3 ORCID: 0000-0002-7589-7823, bogorodskiy173@gmail.com
4 ORCID: 0000-0003-4398-9712, borshchevskiy.vi@ phystech.edu
5 ORCID: 0000-0001-5278-9937, mshevch@gmail.com
Аннотация
Second harmonic generation (SHG) is a nonlinear optical process, and SHG microscopy is a key method for imaging in biomedical science. In the present study, we evaluated the usage of SHG for the imaging of the airways and vessels in the optically cleared mouse lung. SHG visualized F-actin and mainly collagen fibers bordering both airways and vessels. For distinguishing airways from vessels, we used additional staining of the airways with Alexa°Fluor°488-labeled streptavidin and developed the image processing technique for the separation of Alexa°Fluor°488 and SHG signals.
The approach described here allows a high-resolution imaging of anatomic structures of the lung of small animals and can be further used for drug and pathogen detection in experimental disease models.
Ключевые слова: lung vessels, mouse model, tissue optical clearing, second harmonic generation, two-photon microscopy.
